DNA damage and host cell apoptosis at the concomitant drug therapy for dwarf tapeworm infection

Russian Journal of Parasitology
DOI: 10.12737/11776
Article history:
Received  03.12.2014
Accepted 24.03.2015
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DNA damage and host cell apoptosis at the concomitant drug therapy for dwarf tapeworm infection

 

Bekish V. Ya. , Zorina V. V.
Vitebsk State Medical University, Belarus
210023, Belarus, Vitebsk, 27 Frunze prsp., е-mail: bekishvl@tut.by


Abstract

The treatment of experimental hymenolepiasis in larval and imaginal stages of parasite development with praziquantel in combination with indomethacin or ibuprofen and a complex of antioxidant vitamins C, E, β-Carotene is an effective way of protection of the host genome of somatic and generative cells and the full dehelmintization of the host. The most effective way of treatment of hymenolepidosis in 6–11 year-old children is the concomitant therapy with praziquantel applied at a single dose of 25 mg/kg of  body weight plus ibuprofen at a rate of 20 mg/kg of body weight, and a complex of antioxidant vitamins C, E, β-Carotene with selenium (2 additional days).

Praziquantel is administered to adults at the same dose in combination with indomethacin and a complex of antioxidant vitamins C, E, β-Carotene with selenium according to the same regimen. 

Materials and methods

175 male mice СВА at 4–5 month of age and of a weight 18–20 g served as a material for experimental study. The clinical reasoning of a concomitant therapy for hymenolepidosis has been performed at Vitebsk Regional Clinical Hospital of Infectious diseases. Two series of experiments were conducted: the 1st – on laboratory animals, the 2nd – with the involvement of patients with hymenolepidosis and blood donors.

In the first series the impact of hymenolepidosis therapy with praziquantel, phenasal, albendazole, indomethacin, ibuprofen and a complex of antioxidant vitamins C, E, β-Carotene with selenium and their combinations on the results of alkaline single cell gel electrophoresis (SCGE) by N. P. Singh et al. with modification of B. Hellman et al. as well as the intensity of dwarf tapeworm infection (in imaginal stages) were studied.

As a common internationally agreed standard for the estimation of genotoxic effect of environmental factors by the DNA-comet assay we used the «tail moment» which was measured by multiplying the percentage of DNA (fluorescence) in the tail by the length of tail.

To estimate the cytotoxic effect, 100 cells were randomly taken for analysis and the percentage of apoptosis with a small sized nucleus and a large tail dispersed in all directions was determined.

The experiments were conducted on 175 mice divided into two groups. The first group contained 75 mice and was a control group.

The second series was devoted to development of the treatment model for hymenolepidosis in humans using praziquantel with indomethacin or ibuprofen and a complex of antioxidant vitamins C, E, β-Carotene with selenium.

The clinical experiments were conducted on 16 patients with hymenolepidosis, 10 of which (first group) were at the age of 6 to 11 years (6 boys and 4 girls) and 6 patients (second group) – at the age of 25 to 36 years (4 men and 2 women).

Results and discussion

The effect of concomitant therapy (praziquantel, phenasal, albendazole), antioxidant therapy (vitamins C, E, β-Carotene with selenium) on the genome of a host as well as the intensity of hymenolepidosis infection in mice infested by the eggs of dwarf tapeworms in imaginal stages at a dose of 20 eggs/g body weight ( from 11 to 13 days) were studied.

The administration of all drugs and their combinations to the animals of control groups did not lead to a significant increase in tail moment of bone marrow cells and apoptotic cells in comparison with the data of intact control except the administration of phenasal.

Keywords: white mice, human, hymenolepidosis, genome damage, genotoxic and cytotoxic effects.


References

1. Bekish V. J., Bekish J. L. Sostoyanie genoma chozyaina pri gelmintozah [Genomic status of the host at helminthosis]. – Vitebsk: Izd-vo VGMU, 2004.  

2. Dyrnev A. D. Primenenie metoda schelochnogo gel-elektrophoresa izolirovanyh kletok dla otsenki genotoksitseskih svoistv prirodnyh i sintetitcheskih soedinenij [Application of the alkaline single cell gel electrophoresis (SCGE) assay for estimation of genotoxic properties of natural and synthetic compounds]. Metod. rekomendacii. RAMN i RASСНN. – Мoscow, 2006. – 27 p.

3. Hellman B., Vaghef H., Friis L., Edling C. Alkaline single cell gel electrophoresis of DNA fragments in biomonitoring for genotoxicity: an introductory study on healthy human volunteers. Int. Arch. Occup. Environ. Health, 1997, vol. 69, pp.185 –192.

4. Singh N., McCoy M., Tice R., Schneider E. A Simple Technique for quantification of low levels of DNA damage in individual cells. Exp. Cell Research, 1988, vol. 175, pp.184 –191.


© 2015 The Author(s). Published by All-Russian Scientific Research Institute of  Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin. 
This is an open access article under the Agreement of 02.07.2014 (Russian Science Citation Index (RSCI)) and the Agreemnt of 12/06/2014 (CABI org / Human Sciences section)